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This information can guide investigators in choosing the most appropriate sampling method to examine specific physiological states from different sources of RNA during lactation for different research applications. One of the simplest procedures to examine the transcriptome associated with milk appears to be to isolate mRNA directly from MFG and SC that are released into milk during lactation. Our analyses suggest that the SC and MFG transcriptome are the most representative of MGT and LCMEC and can be used as an effective and easy way to study gene expression using RNA Sequencing nfl jerseys, without the need to perform a mammary gland biopsy.MethodsAnimal materialMammary gland tissue and milk samples were obtained from two Jerseys and one Normande cow at the University of California Davis (UCD) and the Institut National de la Recherche Agronomique (INRA) herd (Le Pin au Haras, France), respectively.

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